September 9, 2015
Last week I was in Kathmandu setting up genetic analysis methods for the Himalayan Wolf Project.
The project, which aims to provide a scientific basis for national and international conservation of the Himalayan wolf, is led by Geraldine Werhahn who is a researcher with the University of Oxford’s WildCru. RZSS Wildgenes is partnering with the project by providing design of genetic protocols and training to the Center for Molecular Dynamics Nepal, a laboratory in the capital Kathmandu.
Geraldine has just returned from a two month expedition to the remote Humla Valley where she surveyed the wolves and collected their scats for analysis. In future, surveys will be expanded across the region where wolves are now predominantly confined to remote high valleys. Wolves are threatened by hunting both for protection against livestock loss and for the wildlife trade as their paws are popular talismans.

The survey team on the move through wolf habitat.

Helen and the CMDN team at work testing the new protocols designed by the RZSS WildGenes lab team in the Kathmandu lab.

Discussing some initial results.

Masala chai break with Kathmandu sky line.
Whilst Geraldine has been spending long days at altitude (over 4000m) looking for samples, the WildGenes team has been busy at the lab at RZSS Edinburgh Zoo developing genetic protocols for analysis of the samples. We did this with the help of the keepers from RZSS Highland Wildlife Park who collected scats from our very own grey wolves so that we could test-run the methods.
Once we had the protocols up and running, I could travel to Kathmandu to transfer them to the team at the Center for Molecular Dynamics Nepal who will conduct the bulk of the analysis. We are aiming to use genetic profiling to understand how many wolves there are, what sex they are and how evolutionarily different they are from Eurasian grey wolf.
Dr Helen Senn
RZSS Research Scientist

What it’s all about: The elusive Himalayan wolf.
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